High performance liquid chromatography is an important branch of chromatography. It uses liquid as the mobile phase and uses a high-pressure infusion system to pump single solvents with different polarities or mixed solvents and buffers in different proportions into the stationary phase. The chromatographic column, after the components in the column are separated, enters the detector for detection, so as to realize the analysis of the sample. This method has become an important separation analysis technique in the fields of chemistry, medicine, industry, agronomy, commodity inspection and legal inspection.